Rougeron V., Waleckx E., Hide Malorie, De Meeûs Thierry, Arevalo J., Llanos Cuentas A., Banuls Anne-Laure. (2008). A set of 12 microsatellite loci for genetic studies of Leishmania braziliensis. Molecular Ecology Resources, 8 (2), p. 351-353. ISSN 1471-8278.
Titre du document
A set of 12 microsatellite loci for genetic studies of Leishmania braziliensis
Rougeron V., Waleckx E., Hide Malorie, De Meeûs Thierry, Arevalo J., Llanos Cuentas A., Banuls Anne-Laure
Source
Molecular Ecology Resources, 2008,
8 (2), p. 351-353 ISSN 1471-8278
Twelve microsatellite loci of Leishmania braziliensis were examined, nine of which were developed in this work. Fifty-six Leishmania braziliensis were genotyped with these microsatellite loci. The 12 loci studied were polymorphic with the number of alleles ranging from five to 19, with a mean of 9.7 +/- 4.1 and the observed heterozygosity averaging 0.425 +/- 0.202. The important heterozygote deficits we observed (F-IS = 0.41, P value = 0.004) appear incompatible with the heterozygote excess expected in clonal diploids. This last result could revive the clonality/sexuality debate regarding Leishmania. This work validates the potential use of these microsatellites for population genetics analysis.
