%0 Journal Article %9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES %A Rougeron, V. %A Waleckx, E. %A Hide, Malorie %A De Meeûs, Thierry %A Arevalo, J. %A Llanos Cuentas, A. %A Banuls, Anne-Laure %T A set of 12 microsatellite loci for genetic studies of Leishmania braziliensis %D 2008 %L fdi:010042528 %G ENG %J Molecular Ecology Resources %@ 1471-8278 %K Leishmania braziliensis ; microsatellites ; population genetics ; population structure %M CC:0002538273-0025 %N 2 %P 351-353 %R 10.1111/j.1471-8286.2007.01953.x %U https://www.documentation.ird.fr/hor/fdi:010042528 %> https://www.documentation.ird.fr/intranet/publi/2008/04/010042528.pdf %V 8 %W Horizon (IRD) %X Twelve microsatellite loci of Leishmania braziliensis were examined, nine of which were developed in this work. Fifty-six Leishmania braziliensis were genotyped with these microsatellite loci. The 12 loci studied were polymorphic with the number of alleles ranging from five to 19, with a mean of 9.7 +/- 4.1 and the observed heterozygosity averaging 0.425 +/- 0.202. The important heterozygote deficits we observed (F-IS = 0.41, P value = 0.004) appear incompatible with the heterozygote excess expected in clonal diploids. This last result could revive the clonality/sexuality debate regarding Leishmania. This work validates the potential use of these microsatellites for population genetics analysis. %$ 052