Lequeu J., Simon Plas F., Fromentin J., Etienne P., Petitot Anne-Sophie, Blein J.P., Suty L. (2005). Proteasome comprising a beta 1 inducible subunit acts as a negative regulator of NADPH oxidase during elicitation of plant defense reactions. Febs Letters, 579 (21), p. 4879-4886. ISSN 0014-5793.
Titre du document
Proteasome comprising a beta 1 inducible subunit acts as a negative regulator of NADPH oxidase during elicitation of plant defense reactions
Lequeu J., Simon Plas F., Fromentin J., Etienne P., Petitot Anne-Sophie, Blein J.P., Suty L.
Source
Febs Letters, 2005,
579 (21), p. 4879-4886 ISSN 0014-5793
Elicitation of defense reactions in tobacco by cryptogein, triggered a production of active oxygen species (AOS) via the NADPH oxidase, NtrbohD, and an accumulation of beta 1din, a defense induced beta-type subunit of 20S proteasome. The proteasome inhibitor, MG132, stimulated this AOS production. Tobacco cells transformed with sense constructs of beta 1din showed an inhibition of the AOS production following elicitin treatment, whereas the antisense transformed cells showed a strongly enhanced AOS production. In cells transformed with sense construct of beta 1din, the NtrbohD transcripts failed to be induced by cryptogein as observed in control and antisense transformed cells. Conversely, in tobacco cells transformed with antisense constructs for NtrbohD, Oldin transcripts remained at a low level after elicitation. These results constitute the first demonstration of proteasome comprising Oldin acting as a negative regulator of NtrbohD and contributes to the regulation of AOS generation during plant defense reactions. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
