Callahan D. L., Roessner U., Dumontet V., Perrier Nicolas, Wedd A. G., O' Hair R. A. J., Baker A. J. M., Kolev S. D. (2008). LC-MS and GC-MS metabolite profiling of nickel(II) complexes in the latex of the nickel-hyperaccumulating tree Sebertia acuminata and identification of methylated aldaric acid as a new nickel(II) ligand. Phytochemistry, 69 (1), p. 240-251. ISSN 0031-9422.
Titre du document
LC-MS and GC-MS metabolite profiling of nickel(II) complexes in the latex of the nickel-hyperaccumulating tree Sebertia acuminata and identification of methylated aldaric acid as a new nickel(II) ligand
Callahan D. L., Roessner U., Dumontet V., Perrier Nicolas, Wedd A. G., O' Hair R. A. J., Baker A. J. M., Kolev S. D.
Source
Phytochemistry, 2008,
69 (1), p. 240-251 ISSN 0031-9422
Targeted liquid chromatography mass spectrometry (LC-MS) technology using size exclusion chromatography and metabolite profiling based on gas chromatography-mass spectrometry (GC-MS) were used to study the nickel-rich latex of the hyperaccumulating tree Sebertia acuminata. More than 120 compounds were detected, 57 of these were subsequently identified. A methylated aldaric acid (2,4,5-trihydroxy-3-methoxy-1,6-hexan-dioic acid) was identified for the first time in biological extracts and its structure was confirmed by ID and 2D nuclear magnetic resonance (NMR) spectroscopy. After citric acid, it appears to be one of the most abundant small organic molecules present in the latex studied. Nickel(II) complexes of stoichiometry Ni-II:acid = 1:2 were detected for these two acids as well as for malic, itaconic, erythronic, galacturonic, tartaric, aconitic and saccharic acids. These results provide further evidence that organic acids may play an important role in the transport and possibly in the storage of metal ions in hyperaccumulating plants.
