Callahan D. L. auteur aut IRD Roessner U. auteur aut IRD Dumontet V. auteur aut IRD Perrier Nicolas auteur aut IRD Wedd A. G. auteur aut IRD O' Hair R. A. J. auteur aut IRD Baker A. J. M. auteur aut IRD Kolev S. D. auteur aut IRD text journalArticle eng text/pdf born digital access Targeted liquid chromatography mass spectrometry (LC-MS) technology using size exclusion chromatography and metabolite profiling based on gas chromatography-mass spectrometry (GC-MS) were used to study the nickel-rich latex of the hyperaccumulating tree Sebertia acuminata. More than 120 compounds were detected, 57 of these were subsequently identified. A methylated aldaric acid (2,4,5-trihydroxy-3-methoxy-1,6-hexan-dioic acid) was identified for the first time in biological extracts and its structure was confirmed by ID and 2D nuclear magnetic resonance (NMR) spectroscopy. After citric acid, it appears to be one of the most abundant small organic molecules present in the latex studied. Nickel(II) complexes of stoichiometry Ni-II:acid = 1:2 were detected for these two acids as well as for malic, itaconic, erythronic, galacturonic, tartaric, aconitic and saccharic acids. These results provide further evidence that organic acids may play an important role in the transport and possibly in the storage of metal ions in hyperaccumulating plants. specialized 076 69 1 240-251 2008 0031-9422 https://www.documentation.ird.fr/hor/fdi:010040998 10.1016/j.phytochem.2007.07.001 0031-9422 [F B010040998] https://www.documentation.ird.fr/hor/fdi:010040998 https://www.documentation.ird.fr/intranet/publi/2008/03/010040998.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2008-03-31 2017-08-23 fdi:010040998 fre