Publications des scientifiques de l'IRD

Herrera J. C., D' Hont A., Lashermes Philippe. (2007). Use of fluorescence in situ hybridization as a tool for introgression analysis and chromosome identification in coffee (Coffea arabica L.). Genome, 50 (7), p. 619-626. ISSN 0831-2796.

Titre du document
Use of fluorescence in situ hybridization as a tool for introgression analysis and chromosome identification in coffee (Coffea arabica L.)
Année de publication
2007
Type de document
Article référencé dans le Web of Science WOS:000249430600002
Auteurs
Herrera J. C., D' Hont A., Lashermes Philippe
Source
Genome, 2007, 50 (7), p. 619-626 ISSN 0831-2796
Fluorescence in situ hybridization (FISH) was used to study the presence of alien chromatin in interspecific hybrids and one introgressed line (S.288) derived from crosses between the cultivated species Coffea arabica and the diploid relatives C canephora and C. liberica. In situ hybridization using genomic DNA from C. canephora and C. arabica as probes showed elevated cross hybridization along the hybrid genome, confirming the weak differentiation between parental genomes. According to our genomic in situ hybridization (GISH) data, the observed genomic resemblance between the modern C. canephora genome (C) and the C. canephora-derived subgenome of C. arabica (C-a) appears rather considerable. Poor discrimination between C and Ca chromosomes supports the idea of low structural modifications of both genomes since the C. arabica speciation, at least in the frequency and distribution of repetitive sequences. GISH was also used to identify alien chromatin segments on chromosome spreads of a C liberica-introgressed line of C arabica. Further, use of GISH together with BAC-FISH analysis gave us additional valuable information about the physical localization of the C. liberica fragments carrying the S(H)3 factor involved in resistance to the coffee leaf rust. Overall, our results illustrate that FISH analysis is a complementary tool for molecular cytogenetic studies in coffee, providing rapid localization of either specific chromosomes or alien chromatin in introgressed genotypes derived from diploid species displaying substantial genomic differentiation from C. arabica.
Plan de classement
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010040804]
Identifiant IRD
fdi:010040804
Contact