Publications des scientifiques de l'IRD

Marmey Philippe, Jalloul A., Alhamdia Majd, Assigbetsé Komi, Cacas J. L., Voloudakis A. E., Champion Antony, Clérivet A., Montillet J. L., Nicole Michel. (2007). The 9-lipoxygenase GhLOX1 gene is associated with the hypersensitive reaction of cotton Gossypium hirsutum to Xanthomonas campestris pv malvacearum. Plant Physiology and Biochemistry, 45 (8), p. 596-606. ISSN 0981-9428.

Titre du document
The 9-lipoxygenase GhLOX1 gene is associated with the hypersensitive reaction of cotton Gossypium hirsutum to Xanthomonas campestris pv malvacearum
Année de publication
2007
Type de document
Article référencé dans le Web of Science WOS:000249084800009
Auteurs
Marmey Philippe, Jalloul A., Alhamdia Majd, Assigbetsé Komi, Cacas J. L., Voloudakis A. E., Champion Antony, Clérivet A., Montillet J. L., Nicole Michel
Source
Plant Physiology and Biochemistry, 2007, 45 (8), p. 596-606 ISSN 0981-9428
Hypersensitive reaction (HR) cell death of cotton to the incompatible race 18 from Xanthomonas campestris pathovar malvacearum (Xcm) is associated with 9S-lipoxygenase activity (LOX) responsible for lipid peroxidation. Here, we report the cloning of cotton (Gossypium hirsutum L.) LOX gene (GhLOXI) and the sequencing of its promoter. GhLOXI was found to be highly expressed during Xcm induced HR. Sequence analysis showed that GhLOXI is a putative 9-LOX, and GhLOXI promoter contains SA and JA responsive elements. Investigation on LOX signalisation on cotyledons infiltrated with salicylic acid (SA), or incubated with methyl-jasmonate (MeJA) revealed that both treatments induced LOX activity and GhLOXI gene expression. HR-like symptoms were observed when LOX substrates were then injected in treated (MeJA and SA) cotyledons or when Xcm compatible race 20 was inoculated on MeJA treated cotyledons. Together these results support the fact that GhLOXI encodes a 9 LOX whose activity would be involved in cell death during cotton HR.
Plan de classement
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010040755]
Identifiant IRD
fdi:010040755
Contact