Marmey Philippe auteur aut IRD Jalloul A. auteur aut IRD Alhamdia Majd auteur aut IRD Assigbetsé Komi auteur aut IRD Cacas J. L. auteur aut IRD Voloudakis A. E. auteur aut IRD Champion Antony auteur aut IRD Clérivet A. auteur aut IRD Montillet J. L. auteur aut IRD Nicole Michel auteur aut IRD text journalArticle eng text/pdf born digital access Hypersensitive reaction (HR) cell death of cotton to the incompatible race 18 from Xanthomonas campestris pathovar malvacearum (Xcm) is associated with 9S-lipoxygenase activity (LOX) responsible for lipid peroxidation. Here, we report the cloning of cotton (Gossypium hirsutum L.) LOX gene (GhLOXI) and the sequencing of its promoter. GhLOXI was found to be highly expressed during Xcm induced HR. Sequence analysis showed that GhLOXI is a putative 9-LOX, and GhLOXI promoter contains SA and JA responsive elements. Investigation on LOX signalisation on cotyledons infiltrated with salicylic acid (SA), or incubated with methyl-jasmonate (MeJA) revealed that both treatments induced LOX activity and GhLOXI gene expression. HR-like symptoms were observed when LOX substrates were then injected in treated (MeJA and SA) cotyledons or when Xcm compatible race 20 was inoculated on MeJA treated cotyledons. Together these results support the fact that GhLOXI encodes a 9 LOX whose activity would be involved in cell death during cotton HR. specialized lipoxygenase hypersensitive reaction Xanthomonas campestris Gossypium hirsutum methyl jasmonate salicylic acid 076 45 8 596-606 2007 0981-9428 https://www.documentation.ird.fr/hor/fdi:010040755 10.1016/j.plaphy.2007.05.002 0981-9428 [F B010040755] https://www.documentation.ird.fr/hor/fdi:010040755 https://www.documentation.ird.fr/intranet/publi/2007/10/010040755.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2007-11-06 2022-09-24 fdi:010040755 fre