Lejon V., Jamonneau Vincent, Solano Philippe, Atchade P., Mumba D., Nkoy N., Bebronne N., Kibonja T., Balharbi F., Wierckx A., Boelaert M., Buscher P. (2006). Detection of trypanosome-specific antibodies in saliva, towards non-invasive serological diagnosis of sleeping sickness. Tropical Medicine and International Health, 11 (5), p. 620-627. ISSN 1360-2276.
Titre du document
Detection of trypanosome-specific antibodies in saliva, towards non-invasive serological diagnosis of sleeping sickness
Année de publication
2006
Auteurs
Lejon V., Jamonneau Vincent, Solano Philippe, Atchade P., Mumba D., Nkoy N., Bebronne N., Kibonja T., Balharbi F., Wierckx A., Boelaert M., Buscher P.
Source
Tropical Medicine and International Health, 2006,
11 (5), p. 620-627 ISSN 1360-2276
Objective: The detection of trypanosome-specific antibodies in saliva is technically feasible, and, if clinically validated, could become an attractive option for non-invasive diagnosis of sleeping sickness. We wanted to optimize the test format of an enzyme-linked immunosorbent assay (ELISA)-based antibody detection system. Methods: Different ELISA formats for antibody detection in serum and saliva were developed and standardized. Saliva and serum samples were collected from 78 patient and 128 endemic control samples, and sensitivity and specificity of saliva ELISAs, serum ELISAs and the card agglutination test for trypanosomiasis (CATT), were evaluated. Results: All ELISA formats showed sensitivity and specificity above 90%. Saliva ELISAs showed a similar test performance as serum ELISAs and the CATT on whole blood or serum. Conclusions: This study confirms the potential of trypanosome-specific antibody detection in saliva.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Localisation
Fonds IRD [F B010035629]
Identifiant IRD
fdi:010035629
