Cohuet Anna, Simard Frédéric, Berthomieu A., Raymond M., Fontenille Didier, Weill M. (2002). Isolation and characterization of microsatellite DNA markers in the malaria vector Anopheles funestus. Molecular Ecology Notes, 2 (4), p. 498-500. ISSN 1471-8278.
Titre du document
Isolation and characterization of microsatellite DNA markers in the malaria vector Anopheles funestus
Année de publication
2002
Type de document
Article
Auteurs
Cohuet Anna, Simard Frédéric, Berthomieu A., Raymond M., Fontenille Didier, Weill M.
Source
Molecular Ecology Notes, 2002,
2 (4), p. 498-500 ISSN 1471-8278
Screening of the Anopheles funestus genomic DNA library detected 18 new sequences with dinucleotide tandem repeats. Primers were designed to amplify the loci and 14 out of 18 gave a repeatable and scorable amplification. Deviations from Hardy-Weinberg expectations were tested for each locus in a sample of 30 wild Anopheles funestus females. No heterozygote deficiency was detected for 11 loci of 14, thus revealing the absence of null alleles. The number of alleles per locus ranged from 5 to 15, and observed heterozygosity from 0.13 to 0.85.
