%0 Journal Article %9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES %A Cohuet, Anna %A Simard, Frédéric %A Berthomieu, A. %A Raymond, M. %A Fontenille, Didier %A Weill, M. %T Isolation and characterization of microsatellite DNA markers in the malaria vector Anopheles funestus %D 2002 %L fdi:010029915 %G ENG %J Molecular Ecology Notes %@ 1471-8278 %K PALUDISME ; MOUSTIQUE ; VECTEUR ; ANALYSE GENETIQUE ; POLYMORPHISME GENETIQUE ; MARQUEUR MOLECULAIRE ; TECHNIQUE PCR ; ELECTROPHORESE %K MICROSATELLITE %K AFRIQUE SUBSAHARIENNE %N 4 %P 498-500 %R 10.1046/j.1471-8286.2002.00290.x %U https://www.documentation.ird.fr/hor/fdi:010029915 %> https://www.documentation.ird.fr/intranet/publi/depot/2020-10-16/010029915.pdf %V 2 %W Horizon (IRD) %X Screening of the Anopheles funestus genomic DNA library detected 18 new sequences with dinucleotide tandem repeats. Primers were designed to amplify the loci and 14 out of 18 gave a repeatable and scorable amplification. Deviations from Hardy-Weinberg expectations were tested for each locus in a sample of 30 wild Anopheles funestus females. No heterozygote deficiency was detected for 11 loci of 14, thus revealing the absence of null alleles. The number of alleles per locus ranged from 5 to 15, and observed heterozygosity from 0.13 to 0.85. %$ 052ANOPAL