Vidal Nicole, Peeters Martine, Mulanga Kabeya Claire, Nzilambi N., Robertson D., Ilunga W., Sema H., Tshimanga K., Bongo B., Delaporte Eric. (2000). Unprecedented degree of human immunodeficiency virus type 1 (HIV-1) group M genetic diversity in the Democratic Republic of Congo suggests that the HIV-1 pandemic originated in Central Africa. Journal of Virology, 74 (22), p. 10498-10507. ISSN 0022-538X.
Titre du document
Unprecedented degree of human immunodeficiency virus type 1 (HIV-1) group M genetic diversity in the Democratic Republic of Congo suggests that the HIV-1 pandemic originated in Central Africa
Année de publication
2000
Auteurs
Vidal Nicole, Peeters Martine, Mulanga Kabeya Claire, Nzilambi N., Robertson D., Ilunga W., Sema H., Tshimanga K., Bongo B., Delaporte Eric
Source
Journal of Virology, 2000,
74 (22), p. 10498-10507 ISSN 0022-538X
The purpose of this study was to document the genetic diversity of human immunodeficiency virus type 1 (HIV-1) in the Democratic Republic of Congo (DRC, formerly Zaire). A total of 247 HIV-1-positive samples, collected during an epidemiologic survey conducted in 1997 in three regions (Kinshasa (the capital), Bwamanda (in the north), and Mbuyi-Maya (in the south)), were genetically characterized in the env V3-V5 region. All known subtypes were found to cocirculate, and for 6% of the samples the subtype could not be identified. The highest prevalence (25%) of subtype was in the south, and CRF01-AE was seen mainly in the north. The high intersubtype variability among the V3-V5 sequences is the most probable reason for the low (45%) efficiency of subtype A-specific PCR and HMA (heteroduplex mobility assay). Eighteen (29%) of 62 samples had discordant subtype designations between env and gag. Sequence analysis of the entire envelope from 13 samples confirmed the high degree of diversity and complexity of HIV-1 strains in the DRC ; 9 had a complex recombinant structure in gp160, involving fragments of known and unknown subtypes. Interestingly, the unknown fragments from the different strains did not cluster together. Overall, the high number of HIV-1 subtypes cocirculating, the high intrasubtype diversity, and the high numbers of possible recombinant viruses as well as different unclassified strains are all in agreement with an old and mature epidemic in the DRC, suggesting that this region is the epicenter of HIV-1 group M. (Résumé d'auteur)
Plan de classement
Maladies sexuellement transmissibles [052MALTRA03]
Descripteurs
SIDA ; VIRUS ; DIVERSITE GENETIQUE ; PREVALENCE ; ANALYSE GENETIQUE ; EPIDEMIOLOGIE ; PHYLOGENIE ; REPARTITION GEOGRAPHIQUE ; VIH 1 GROUPE M
Description Géographique
ZAIRE
Localisation
Fonds IRD [F B010025698] ;
Montpellier (Centre IRD)
Identifiant IRD
fdi:010025698
