Mazars E., Lesjean S., Banuls Anne-Laure, Gilbert M., Vincent V., Gicquel B., Tibayrenc Michel, Locht C., Supply P. (2001). High-resolution minisatellite-based typing as a portable approach to global analysis of Mycobacterium tuberculosis molecular epidemiology. Proceedings of the National Academy of Sciences of the United States of America, 98 (4), p. 1901-1906. ISSN 0027-8424.
Titre du document
High-resolution minisatellite-based typing as a portable approach to global analysis of Mycobacterium tuberculosis molecular epidemiology
Année de publication
2001
Auteurs
Mazars E., Lesjean S., Banuls Anne-Laure, Gilbert M., Vincent V., Gicquel B., Tibayrenc Michel, Locht C., Supply P.
Source
Proceedings of the National Academy of Sciences of the United States of America, 2001,
98 (4), p. 1901-1906 ISSN 0027-8424
The worldwide threat of tuberculosis to human health emphasizes the need to develop novel approaches to a global epidemiological surveillance. The current standard for Mycobacterium tuberculosis typing based on IS6110 restriction fragment length polymorphism (RFLP) suffers from the difficulty of comparing data between independent laboratories. Here, we propose a high-resolution typing method based on variable number tandem repeats (VNTRs) of genetic elements named mycobacterial interspersed repetitive units (MIRUs) in 12 human minisatellite-like regions of the M. tuberculosis genome. MIRU-VNTR profiles of 72 different M. tuberculosis isolates were established by PCR analysis of all 12 loci. From 2 to 8 MIRU-VNTR alleles were identified in the 12 regions in these strains, which corresponds to a potential of over 16 million different combinations, yielding a resolution power close to that of IS6110-RFLP. All epidemiologically related isolates tested were perfectly clustered by MIRU-VNTR typing, indicating that the stability of these MIR-VNTRs is adequate to track outbreak episodes. The correlation between genetic relationships inferred from MIRU-VNTR and IS6110-RFLP typing was highly significant. Compared with IS6110-RFLP, high-resolution MIRU-VNTR typing has the considerable advantages of being fast, appropriate for all M. tuberculosis isolates, including strains that have a few IS6110 copies, and permitting easy and rapid comparison of results from independent laboratories. This typing method opens the way to the construction of digital global databases for molecular epidemiology studies of M. tuberculosis. (Résumé d'auteur)
Plan de classement
Divers [052MALTRA05]
Descripteurs
MALADIE ; BACTERIE ; SURVEILLANCE EPIDEMIOLOGIQUE ; METHODE D'ANALYSE ; TECHNIQUE PCR ; TECHNIQUE RFLP ; DIVERSITE GENETIQUE ; ETUDE EXPERIMENTALE ; ETUDE COMPARATIVE ; TUBERCULOSE
Description Géographique
MONDE
Localisation
Fonds IRD [F B010025193] ;
Montpellier (Centre IRD)
Identifiant IRD
fdi:010025193
