@article{fdi:010025193,
  title = {{H}igh-resolution minisatellite-based typing as a portable approach to global analysis of {M}ycobacterium tuberculosis molecular epidemiology},
  author = {{M}azars, {E}. and {L}esjean, {S}. and {B}anuls, {A}nne-{L}aure and {G}ilbert, {M}. and {V}incent, {V}. and {G}icquel, {B}. and {T}ibayrenc, {M}ichel and {L}ocht, {C}. and {S}upply, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he worldwide threat of tuberculosis to human health emphasizes the need to develop novel approaches to a global epidemiological surveillance. {T}he current standard for #{M}ycobacterium tuberculosis$ typing based on {IS}6110 restriction fragment length polymorphism ({RFLP}) suffers from the difficulty of comparing data between independent laboratories. {H}ere, we propose a high-resolution typing method based on variable number tandem repeats ({VNTR}s) of genetic elements named mycobacterial interspersed repetitive units ({MIRU}s) in 12 human minisatellite-like regions of the #{M}. tuberculosis$ genome. {MIRU}-{VNTR} profiles of 72 different #{M}. tuberculosis$ isolates were established by {PCR} analysis of all 12 loci. {F}rom 2 to 8 {MIRU}-{VNTR} alleles were identified in the 12 regions in these strains, which corresponds to a potential of over 16 million different combinations, yielding a resolution power close to that of {IS}6110-{RFLP}. {A}ll epidemiologically related isolates tested were perfectly clustered by {MIRU}-{VNTR} typing, indicating that the stability of these {MIR}-{VNTR}s is adequate to track outbreak episodes. {T}he correlation between genetic relationships inferred from {MIRU}-{VNTR} and {IS}6110-{RFLP} typing was highly significant. {C}ompared with {IS}6110-{RFLP}, high-resolution {MIRU}-{VNTR} typing has the considerable advantages of being fast, appropriate for all #{M}. tuberculosis$ isolates, including strains that have a few {IS}6110 copies, and permitting easy and rapid comparison of results from independent laboratories. {T}his typing method opens the way to the construction of digital global databases for molecular epidemiology studies of #{M}. tuberculosis$. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{MALADIE} ; {BACTERIE} ; {SURVEILLANCE} {EPIDEMIOLOGIQUE} ; {METHODE} {D}'{ANALYSE} ; {TECHNIQUE} {PCR} ; {TECHNIQUE} {RFLP} ; {DIVERSITE} {GENETIQUE} ; {ETUDE} {EXPERIMENTALE} ; {ETUDE} {COMPARATIVE} ; {TUBERCULOSE} ; {MONDE}},
  booktitle = {},
  journal = {{P}roceedings of the {N}ational {A}cademy of {S}ciences of the {U}nited {S}tates of {A}merica},
  volume = {98},
  numero = {4},
  pages = {1901--1906},
  ISSN = {0027-8424},
  year = {2001},
  DOI = {10.1073/pnas.98.4.1901},
  URL = {https://www.documentation.ird.fr/hor/fdi:010025193},
}