Toure-Kane C., Montavon Céline, Faye M.A., Gueye P.M., Sow P.S., Ndoye I., Gaye-Diallo A., Delaporte Eric, Peeters Martine, Mboup S. (2000). Identification of all HIV type 1 group M subtypes in Senegal, a country with low and stable seroprevalence. AIDS Research and Human Retroviruses, 16 (6), p. 603-609. ISSN 0889-2229.
Titre du document
Identification of all HIV type 1 group M subtypes in Senegal, a country with low and stable seroprevalence
Année de publication
2000
Auteurs
Toure-Kane C., Montavon Céline, Faye M.A., Gueye P.M., Sow P.S., Ndoye I., Gaye-Diallo A., Delaporte Eric, Peeters Martine, Mboup S.
Source
AIDS Research and Human Retroviruses, 2000,
16 (6), p. 603-609 ISSN 0889-2229
A total of 343 HIV-1-positive samples obtained between June 1996 and March 1999 was genetically characterized in the envelop region by HMA and/or sequencing. The env subtype distribution was as follows : 290 (84.6%) A, 22 (6.5%) B, 16 (4.7%) C, 8 (2.5%) D, 1 (0.03%) E, 1 (0.03%) F1, 4 (1.2%) G, and 1 (0.03%) H. For 77 samples the p24 region from the gag gene was also sequenced, and for 9 (11.6%) the subtypes between env and gag were different. Phylogenetic tree analysis showed the predominance of AG-IBNG-like viruses among gag and env subtype A sequences. HMA is relatively simple and requires less sophisticated technical facilities compared with sequencing, and in Senegal 323 (94.2%) of the 343 samples could be identified by this technique. However, in the actual configuration of the assay, discrimination between the recombinant AG-IBNG-like recombinant viruses, which are predominant in Senegal, and the nonrecombinant subtype A viruses is not possible. (Résumé d'auteur)
Plan de classement
Maladies sexuellement transmissibles [052MALTRA03]
Descripteurs
SIDA ; VIRUS ; PREVALENCE ; ANALYSE GENETIQUE ; TECHNIQUE PCR ; ETUDE EXPERIMENTALE ; HMA.HETERODUPLEX MOBILITY ASSAY
Description Géographique
SENEGAL ; DAKAR
Localisation
Fonds IRD [F B010024062] ;
Montpellier (Centre IRD)
Identifiant IRD
fdi:010024062
