%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Toure-Kane, C.
%A Montavon, Céline
%A Faye, M.A.
%A Gueye, P.M.
%A Sow, P.S.
%A Ndoye, I.
%A Gaye-Diallo, A.
%A Delaporte, Eric
%A Peeters, Martine
%A Mboup, S.
%T Identification of all HIV type 1 group M subtypes in Senegal, a country with low and stable seroprevalence
%D 2000
%L fdi:010024062
%G ENG
%J AIDS Research and Human Retroviruses
%@ 0889-2229
%K SIDA ; VIRUS ; PREVALENCE ; ANALYSE GENETIQUE ; TECHNIQUE PCR ; ETUDE EXPERIMENTALE
%K HMA.HETERODUPLEX MOBILITY ASSAY
%K SENEGAL ; DAKAR
%N 6
%P 603-609
%R 10.1089/088922200309025
%U https://www.documentation.ird.fr/hor/fdi:010024062
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_57-58/010024062.pdf
%V 16
%W Horizon (IRD)
%X A total of 343 HIV-1-positive samples obtained between June 1996 and March 1999 was genetically characterized in the envelop region by HMA and/or sequencing. The env subtype distribution was as follows : 290 (84.6%) A, 22 (6.5%) B, 16 (4.7%) C, 8 (2.5%) D, 1 (0.03%) E, 1 (0.03%) F1, 4 (1.2%) G, and 1 (0.03%) H. For 77 samples the p24 region from the gag gene was also sequenced, and for 9 (11.6%) the subtypes between env and gag were different. Phylogenetic tree analysis showed the predominance of AG-IBNG-like viruses among gag and env subtype A sequences. HMA is relatively simple and requires less sophisticated technical facilities compared with sequencing, and in Senegal 323 (94.2%) of the 343 samples could be identified by this technique. However, in the actual configuration of the assay, discrimination between the recombinant AG-IBNG-like recombinant viruses, which are predominant in Senegal, and the nonrecombinant subtype A viruses is not possible. (Résumé d'auteur)
%$ 052MALTRA03