Chandre Fabrice, Darriet Frédéric, Duchon S., Finot Luc, Manguin Sylvie, Carnevale Pierre, Guillet Pierre. (2000). Modifications of pyrethroid effects associated with kdr mutation in Anopheles gambiae. Medical and Veterinary Entomology, 14 (1), p. 81-88. ISSN 0269-283X.
Titre du document
Modifications of pyrethroid effects associated with kdr mutation in Anopheles gambiae
Année de publication
2000
Auteurs
Chandre Fabrice, Darriet Frédéric, Duchon S., Finot Luc, Manguin Sylvie, Carnevale Pierre, Guillet Pierre
Source
Medical and Veterinary Entomology, 2000,
14 (1), p. 81-88 ISSN 0269-283X
Effects of knockdown resistance (kdr) were investigated in three pyrethroid-resistant (PR) strains of the Afrotropical mosquito Anopheles gambiae Giles (Diptera : Culicidae) : Kou from Burkina Faso, Tola and Yao from Côte d'Ivoire ; compared with a standard susceptible (SS) strain from Kisumu, Kenya. The kdr factor was incompletly recessive, conferring 43-fold resistance ratio at LD50 level and 29-fold at LD95 level, as determinated by topical application tests with Kou strain. When adult mosquitoes were exposed to 0.25% permethrine-impregnated papers, the 50% and 95% knockdown times (KdT) were 23 and 42 min for SS females, compared with 40 and 62 min for RS (F1 Kou x Kisumu) females. On 1% permethrin the kdt50 and kdt95 were 11 and 21 min for SS compared with 18 and 33 min for RS females. Following 1 h exposure to permethrin (0.25% or 1%), no significant knockdown of Kou RR females occurred within 24 h. Permethrin irritancy to An. gambiae was assessed by comparing "time to first take-off" (TO) for females. The standard TO50 and TO95 values for Kisumu SS on untreated paper were 58 and 1044s, respectively, vs. 3.7 and 16.5s on 1% permethrin. For Kou RR females the comparable values were 27.3s for TO50 and 294s for TO95, with intermediate RS values of 10.1s for TO50 and 71.9s for TO95. Thus, TO values for RS were 2.7-4.4 times more than for SS, and those for RR were 7-18 times longer than for SS. Experiments with pyrethroid-impregnated nets were designed to induce hungry female mosquitoes to pass through holes cut in the netting. Laboratory "tunnels tests" used a bait guinea-pig to attract mosquitoes through circular holes (5 x 1 cm) in a net screen. With untreated netting, 75-83% of laboratory-reared females passed through the holes overnight, 63-69% blood-fed successfully and 9-17% died, with no significant differences between SS and RR genotypes... (D'après résumé d'auteur)
Plan de classement
Lutte [052ANOPAL04]
Descripteurs
PALUDISME ; VECTEUR ; MOUSTIQUE ; METHODE DE LUTTE ; MOUSTIQUAIRE IMPREGNEE ; INSECTICIDE CHIMIQUE ; SENSIBILITE RESISTANCE ; GENE ; MUTATION ; ETUDE COMPARATIVE ; PERMETHRINE ; DELTAMETHRINE ; GENE KDR
Description Géographique
COTE D'IVOIRE ; BURKINA FASO
Localisation
Fonds IRD [F B010022274] ;
Montpellier (Centre IRD)
Identifiant IRD
fdi:010022274
