Triques Karine, Bourgeois A., Vidal Nicole, Mpoudi Ngolé E., Mulanga Kabeya Claire, Nzilambi N., Torimiro N., Saman E., Delaporte Eric, Peeters Martine. (2000). Near-full-length genome sequencing of divergent African HIV type 1 subtype F viruses leads to the identification of a new HIV type 1 subtype designated K. AIDS Research and Human Retroviruses, 16 (2), p. 139-151. ISSN 0889-2229.
Titre du document
Near-full-length genome sequencing of divergent African HIV type 1 subtype F viruses leads to the identification of a new HIV type 1 subtype designated K
Année de publication
2000
Auteurs
Triques Karine, Bourgeois A., Vidal Nicole, Mpoudi Ngolé E., Mulanga Kabeya Claire, Nzilambi N., Torimiro N., Saman E., Delaporte Eric, Peeters Martine
Source
AIDS Research and Human Retroviruses, 2000,
16 (2), p. 139-151 ISSN 0889-2229
We recently reported a high divergence among African subtype F strains. Three well-separated groups (F1, F2, and F3) have been shown based on the phylogenetic analysis of the p24 gag and envelope sequences with genetic distances similar to those observed for known subtypes. In this study, we characterized the near-full-length genomes of two strains from epidemiological unlinked individual belonging to each of the subgroups : F1 (96FR-MP411), F2 (95CM-MP255 and 95CM-MP257), and F3 (96CM-MP535 and 97ZR-EQTB11). Phylogenetic analysis of the near-full-length sequences and for each of the genes separately showed the same three groups, supported by high bootstrap values. Diversity plotting, BLAST subtyping, and bootstrap plotting confirmed that the divergent F strains correspond to nonrecombinant viruses. The divergence between F1 and F2 is consistently lower than that seen in any other intersubtype comparison, with the exception of subtypes B and D. Based on all the different analyses, we propse to divide subtype F into two subclades, with F1 gathering the known subtype strains from Brazil and Finland, and our African strain (96FR-MP411), and F2 containing the 95CM-MP255 and 95CM-MP257 strains from Cameroon. The F3 strains, 97ZR-EQTB11 from the Democratic Republic of Congo and 96CM-MP535 from Cameroon, meet the criteria of a new subtype designated as K. The equidistance of subtype K to the other subtypes of HIV-1 suggests that this subtype existed as long as the others, the lower distance between B and D, and between F1 and F2 suggest a more recent subdivision for these latter strains. (Résumé d'auteur)
Plan de classement
Maladies sexuellement transmissibles [052MALTRA03]
Descripteurs
SIDA ; VIRUS ; STRUCTURE GENETIQUE ; GENOME ; PHYLOGENIE ; ANALYSE GENETIQUE ; VARIABILITE GENETIQUE ; DISTANCE GENETIQUE ; TECHNIQUE PCR ; ETUDE EXPERIMENTALE ; ETUDE COMPARATIVE ; RECOMBINAISON GENETIQUE
Description Géographique
AFRIQUE ; CAMEROUN ; CONGO ; TCHAD ; YOUGOSLAVIE
Localisation
Fonds IRD [F B010021755] ;
Montpellier (Centre IRD)
Identifiant IRD
fdi:010021755
