Pintado J., Guyot Jean-Pierre, Raimbault Maurice. (1999). Lactic acid production from mussel processing wastes with an amylolytic bacterial strain. Enzyme and Microbial Technology, 24, p. 590-598. ISSN 0141-0229.
Titre du document
Lactic acid production from mussel processing wastes with an amylolytic bacterial strain
Année de publication
1999
Type de document
Article
Auteurs
Pintado J., Guyot Jean-Pierre, Raimbault Maurice
Source
Enzyme and Microbial Technology, 1999,
24, p. 590-598 ISSN 0141-0229
The aim of this work was to study the capability of amylolytic lactic acid bacteria to hydrolyse the glycogen in mussel processing wastes (MPW) for lactic acid production. From four strains tested, Lactobacillus plantarum A6 was selected. Cultures of this strain on MRS medium with starch or mussel glycogen, showed similar levels of amylase production for both polysaccharides. With glycogen, sugar comsumption rate was lower than with soluble starch and, although the micro-organism produced higher yields of enzyme, a fraction of approximately 20% of the total sugars remained undergraded. However, the different substrates did not seem to induce the production of different types of amylase and the values of the apparent Km for starch and for glycogen were close, revealing a similar affinity for both substrates. Therefore, the differences in hydrolysis and consumption of the substrate seem to be related with the higher grade of branching of glycogen. Culture in MPW showed a decrease in pH unfavourable for amylolytic activity and uncoupling between lactic acid and biomass production. By controlling pH and increasing the initial protein concentration to avoid uncoupling, similar lactic acid production and higher yields than obtained with starch were reached with MPW. (Résumé d'auteur)
Plan de classement
Aérobies [084FERMEN01]
Descripteurs
BIOTECHNOLOGIE ; FERMENTATION ; DECHET AGROINDUSTRIEL ; BACTERIE LACTIQUE ; ACTIVITE ENZYMATIQUE ; MILIEU DE CULTURE ; RECYCLAGE DES DECHETS ; RENDEMENT ; EFFLUENT ; ACIDE LACTIQUE ; AMYLASE
