%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Pintado, J.
%A Guyot, Jean-Pierre
%A Raimbault, Maurice
%T Lactic acid production from mussel processing wastes with an amylolytic bacterial strain
%D 1999
%L fdi:010018317
%G ENG
%J Enzyme and Microbial Technology
%@ 0141-0229
%K BIOTECHNOLOGIE ; FERMENTATION ; DECHET AGROINDUSTRIEL ; BACTERIE LACTIQUE ; ACTIVITE ENZYMATIQUE ; MILIEU DE CULTURE ; RECYCLAGE DES DECHETS ; RENDEMENT
%K EFFLUENT ; ACIDE LACTIQUE ; AMYLASE
%P 590-598
%R 10.1016/S0141-0229(98)00168-9
%U https://www.documentation.ird.fr/hor/fdi:010018317
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_49-50/010018317.pdf
%V 24
%W Horizon (IRD)
%X The aim of this work was to study the capability of amylolytic lactic acid bacteria to hydrolyse the glycogen in mussel processing wastes (MPW) for lactic acid production. From four strains tested, #Lactobacillus plantarum$ A6 was selected. Cultures of this strain on MRS medium with starch or mussel glycogen, showed similar levels of amylase production for both polysaccharides. With glycogen, sugar comsumption rate was lower than with soluble starch and, although the micro-organism produced higher yields of enzyme, a fraction of approximately 20% of the total sugars remained undergraded. However, the different substrates did not seem to induce the production of different types of amylase and the values of the apparent Km for starch and for glycogen were close, revealing a similar affinity for both substrates. Therefore, the differences in hydrolysis and consumption of the substrate seem to be related with the higher grade of branching of glycogen. Culture in MPW showed a decrease in pH unfavourable for amylolytic activity and uncoupling between lactic acid and biomass production. By controlling pH and increasing the initial protein concentration to avoid uncoupling, similar lactic acid production and higher yields than obtained with starch were reached with MPW. (Résumé d'auteur)
%$ 084FERMEN01