V3 serotyping of HIV-1 infection : correlation with genotyping and limitations

1999

Article

Plantier J.C., Damond F., Lasky Mariama, Sankalé J.L., Apetrei C., Peeters Martine, Buzelay L., M'Boup S., Kanki P., Delaporte Eric, Simon F., Barin F.

Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, 1999, 20 (5), p. 432-441 ISSN 1077-9450

HIV-1 V3 serotyping is a classification of immunodeficiency viruses based on antibody binding to V3 peptides that allows obtaining information on circulating subtypes that could be important for population-based epidemiologic studies. Recently, several laboratories have developed V3 enzyme-immunoassays (EIAs) using V3 peptides of subtypes A to E. In the present study, the utility of including additional peptides of subtypes F to H to the EIA was evaluated on a panel of 203 well-characterized serum samples from patents with diverse geographic origins (22 countires) and known HIV-1 genotype (79 A, 61 B, 21 C, 7 D, 7 E, 21 F, 6 G, 1 H). The results indicate a high predictive value (ppv) for serotypes B (greater than or equal to 0.86), D (1) and E (0.88), and confirm the difficulty of predicting genotype A or C based on serotype A or C. Results also indicate that inclusion of the F peptide in the V3 EIAs may be useful (ppv = 0.61), but introduction of peptides G and H failed to demonstrate significant sensitivity or specificity for these subtypes. Correlation between serotyping and amino-acid sequences of the V3 region from 103 samples allowed the identification of key amino-acids that appear essential for subtype-specific seroreactivity. (Résumé d'auteur)

Maladies sexuellement transmissibles [052MALTRA03]

SIDA ; VIRUS ; TYPOLOGIE ; ANTICORPS ; CLASSIFICATION ; SEROLOGIE ; TEST ; IMMUNOLOGIE ; GENOTYPE ; SEROTYPAGE ; PEPTIDE V3

Fonds IRD [F B010018170]

fdi:010018170