Bourguet D., Fonseca D., Vourch G., Dubois M.P., Chandre Fabrice, Severini C., Raymond M. (1998). The acetylcholinesterase gene ACE : a diagnostic marker for the pipiens and quinquefasciatus forms of the Culex pipiens complex. Journal of the American Mosquito Control Association, 14 (4), p. 390-396. ISSN 8756-971X.
Titre du document
The acetylcholinesterase gene ACE : a diagnostic marker for the pipiens and quinquefasciatus forms of the Culex pipiens complex
Année de publication
1998
Type de document
Article
Auteurs
Bourguet D., Fonseca D., Vourch G., Dubois M.P., Chandre Fabrice, Severini C., Raymond M.
Source
Journal of the American Mosquito Control Association, 1998,
14 (4), p. 390-396 ISSN 8756-971X
The taxonomy of the Culex pipiens complex remains a controversial issue in mosquito systematics. Based on morphologic characters, 2 allopatric taxa are recognized, namely Cx. pipiens (including the form "molestus") in temperate areas and Cx. quinquefasciatus in tropical areas. Here we report on variability at nucleotide level of an acetylcholinesterase gene in several strains and natural populations of this species complex. Few polymorphisms were found in coding regions within a subspecies but many polymorphisms were observed between subspecies in noncoding regions. We describe a method based on a restriction enzyme polymorphism in polmerase chain reaction-amplified DNA, in which the presence or absence of one restriction site discriminates Cx. pipiens, Cx. quinquefasciatus, and their hybrids. This technique reliably discriminates mosquitoes from more than 30 worldwide strains or populations. Polymerase chain reaction amplification of specific alleles may also be a useful tool for characterizing specific alleles of each sibling taxon. (Résumé d'auteur)
