Publications des scientifiques de l'IRD

Yamèogo L., Toè L., Hougard Jean-Marc, Boatin B.A., Unnasch T.R. (1999). Pool screen polymerase chain reaction for estimating the prevalence of Onchocerca volvulus infection in Simulium damnosum sensu lato : results of a field trial in an area subject to successful vector control. American Journal of Tropical Medicine and Hygiene, 60 (1), p. 124-128. ISSN 0002-9637.

Titre du document
Pool screen polymerase chain reaction for estimating the prevalence of Onchocerca volvulus infection in Simulium damnosum sensu lato : results of a field trial in an area subject to successful vector control
Année de publication
1999
Type de document
Article
Auteurs
Yamèogo L., Toè L., Hougard Jean-Marc, Boatin B.A., Unnasch T.R.
Source
American Journal of Tropical Medicine and Hygiene, 1999, 60 (1), p. 124-128 ISSN 0002-9637
Detection of infective parasites in the vector population can be an early indicator of recrudescence in areas freed of new cases of onchocerciasis. However, dissection of vector black flies is inefficient in areas subject to effective control. Recently, a polymerase chain reaction (PCR)-based assay has been used to detect a single Onchocerca volvulus-infected black fly in pools containing large numbers of uninfected flies. This method had not been validated on wild-caught black flies in an area subject to effective vector control. Here, we report a method of restricting the pool screen PCR assay to infectious parasites and the results of a field test in an area subject to long-term vector control. The prevalence of infection determined by dissection did not differ from that determined by pool screen PCR. The results suggest that the PCR assay may be a useful tool for epidemiologic surveillance for O. volvulus infection. (Résumé d'auteur)
Plan de classement
Lutte [052SIMONC04]
Descripteurs
VECTEUR ; PARASITE ; INFECTION ; ESTIMATION ; TECHNIQUE PCR ; ETUDE COMPARATIVE ; SURVEILLANCE ENTOMOLOGIQUE
Description Géographique
AFRIQUE DE L'OUEST
Localisation
Fonds IRD [F B010017862] ; Montpellier (Centre IRD)
Identifiant IRD
fdi:010017862
Contact