@article{fdi:010017862,
  title = {{P}ool screen polymerase chain reaction for estimating the prevalence of {O}nchocerca volvulus infection in {S}imulium damnosum sensu lato : results of a field trial in an area subject to successful vector control},
  author = {{Y}am{\`e}ogo, {L}. and {T}o{\`e}, {L}. and {H}ougard, {J}ean-{M}arc and {B}oatin, {B}.{A}. and {U}nnasch, {T}.{R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{D}etection of infective parasites in the vector population can be an early indicator of recrudescence in areas freed of new cases of onchocerciasis. {H}owever, dissection of vector black flies is inefficient in areas subject to effective control. {R}ecently, a polymerase chain reaction ({PCR})-based assay has been used to detect a single #{O}nchocerca volvulus$-infected black fly in pools containing large numbers of uninfected flies. {T}his method had not been validated on wild-caught black flies in an area subject to effective vector control. {H}ere, we report a method of restricting the pool screen {PCR} assay to infectious parasites and the results of a field test in an area subject to long-term vector control. {T}he prevalence of infection determined by dissection did not differ from that determined by pool screen {PCR}. {T}he results suggest that the {PCR} assay may be a useful tool for epidemiologic surveillance for #{O}. volvulus$ infection. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{VECTEUR} ; {PARASITE} ; {INFECTION} ; {ESTIMATION} ; {TECHNIQUE} {PCR} ; {ETUDE} {COMPARATIVE} ; {SURVEILLANCE} {ENTOMOLOGIQUE} ; {AFRIQUE} {DE} {L}'{OUEST}},
  booktitle = {},
  journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene},
  volume = {60},
  numero = {1},
  pages = {124--128},
  ISSN = {0002-9637},
  year = {1999},
  URL = {https://www.documentation.ird.fr/hor/fdi:010017862},
}