Léry Xavier, Khamiss O., Nour-El-Din A., Giannotti J., Abol-Ela S. (1997). Multiplication of Spodoptera littoralis granulosis virus in a cell line established from Phthorimaea operculella. Acta Virologica, 41, p. 169-174. ISSN 0001-723X.
Titre du document
Multiplication of Spodoptera littoralis granulosis virus in a cell line established from Phthorimaea operculella
Année de publication
1997
Type de document
Article
Auteurs
Léry Xavier, Khamiss O., Nour-El-Din A., Giannotti J., Abol-Ela S.
Source
Acta Virologica, 1997,
41, p. 169-174 ISSN 0001-723X
A complete replication of the Spodoptera littoralis granulosis virus (SpliGV) was obtained in vitro by both virus infection and DNA transfection in the ORS-Pop-95 (Pop-95) cell line established from embryonic cells of the potato tuber moth, Phthorimaea operculella. SpliGV multiplied significantly during several passages in Pop-95 cells at 19°C. When the cells were infected and kept at 19°C for the first 4 hrs and then at 27°C for the rest of the experiment (20 days), the viral multiplication proceeded at the same rate. Comparison of SpliGV progenies, multiplied either in vivo or in vitro, using electron microscopy and restiction profile analysis, showed their identity. (Résumé d'auteur)
