Brisse Sylvain, Banuls Anne-Laure, Sidibé Issa, Barnabé Christian, Tibayrenc Michel. (1996). Genetic structure and evolution of Trypanosoma cruzi, the agent of Chagas disease, as revealed by multiprimer RAPD analysis : clonality vs sexuality. Memorias do Instituto Oswaldo Cruz, 91 (no spécial), p. 277. ISSN 0074-0276.
Titre du document
Genetic structure and evolution of Trypanosoma cruzi, the agent of Chagas disease, as revealed by multiprimer RAPD analysis : clonality vs sexuality
Année de publication
1996
Type de document
Article
Auteurs
Brisse Sylvain, Banuls Anne-Laure, Sidibé Issa, Barnabé Christian, Tibayrenc Michel
Source
Memorias do Instituto Oswaldo Cruz, 1996,
91 (no spécial), p. 277 ISSN 0074-0276
Trypanosoma cruzi natural populations have been shown to be highly heterogeneous and subdivided into a number of genetically distant clonal lineages. We have selected 21 highly resolutive primers, out of 120 tested, to analyse 50 stocks representative of the major genetic subdivisions of the parasite. Stocks were isolated from diverse hosts and from different geographic areas. Other trypanosomatids, including Leishmania and T. congolense, were analysed in parallel in order to directly compare their genetic diversity with T. cruzi. The results show the marked genetic structuration of T. cruzi in a few distinct genetic groups, confirming previous MLEE and RAPD data. These groups are interpreted as clonal lineages. RAPD markers specific for each group are numerous and will be used to develop probes for specific diagnostic purposes. The huge genetic heterogeneity of T. cruzi is confirmed by the divergence between lineages of this parasite which is similar to that observed between species, and even species complexes, within the Leishmania genus. The phylogenetic relationships between the T. cruzi groups were investigated by multivariate and phylogenetic analysis. The monophyly of each group appears robust, but the hierarchical relationships between groups are problematic. Moreover, some groups display features that could be interpreted as the result of a hybridization event between strains of two other groups. These findings suggest the possibility of an evolutionary process involving both occasional recombination leading to new genotypes and clonal evolution of successful lineages. (Résumé d'auteur)
Plan de classement
Epidémiologie des trypanosomiases [052GLOTRY02]
Descripteurs
MALADIE DE CHAGAS ; AGENT PATHOGENE ; POLYMORPHISME GENETIQUE ; CLONE ; RAPD.RANDOM AMPLIFIED POLYMORPHIC DNA
