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Jmeii L., Soufi L., Abid N., Mahjoubi M., Roussos Sevastianos, Ouzari H. I., Cherif A., Garna H. (2019). Assessment of biotechnological potentials of strains isolated from repasso olive pomace in Tunisia. Annals of Microbiology, 69 (11), 1177-1190. ISSN 1590-4261

Lien direct chez l'éditeur doi:10.1007/s13213-019-01499-y

Titre
Assessment of biotechnological potentials of strains isolated from repasso olive pomace in Tunisia
Année de publication2019
Type de documentArticle référencé dans le Web of Science WOS:000505158100007
AuteursJmeii L., Soufi L., Abid N., Mahjoubi M., Roussos Sevastianos, Ouzari H. I., Cherif A., Garna H.
SourceAnnals of Microbiology, 2019, 69 (11), p. 1177-1190. ISSN 1590-4261
RésuméPurpose The agri-food industry generates significant amounts of byproducts, among them repasso olive pomace (ROP) which are rejected and can constitute a serious environmental problem. Our study aimed to investigate the diversity of microbiota isolated from ROP and screen for their biotechnological potentials. Methods A collection of 102 strains (88 bacteria and 14 fungi) was obtained from a ROP sample. The diversity of the bacterial collection was evaluated by amplification of the internal transcribed spacers between the 16S and the 23S rRNA genes (ITS-PCR) and by 16S rRNA sequencing. Fungal identification was performed by polymerase chain reaction (PCR) amplification of the ITS1-5.8S-ITS2 ribosomal DNA region. The specific enzymatic screening of the detected microorganisms was tested. Result Partial 16S rRNA gene sequencing performed on 44 isolates showed a high level of identity with known strains. Fungal strains identification showed that they belong to four families: Trichocomaceae, Pleurostomataceae, Mucoraceae, and Bionectriaceae. Our results demonstrated that Gram-positive bacteria were mostly active, particularly for protease, lipase, amylase, cellulase laccase, and for biosurfactant production. From the 88 isolated bacteria, Firmicutes were the most prevalent and microdiverse. Bacillus and Paenibacillus, together with some other Gram-negative bacteria such as Pseudocitrobacter anthropi, and Acinetobacter johnsonii showed significant hydrolytic activities and biosurfactant production. The 14 isolated fungi showed a high capacity of enzyme production. This is the first study in Tunisia describing the microbial diversity in ROP as well as the isolation of Bacillus mojavensis producing lipase. Conclusion Microorganisms especially fungi present in the repasso olive cake produce diverse hydrolytic enzymes of industrial interest.
Plan de classementBiotechnologies [084]
Descr. géo.TUNISIE
LocalisationFonds IRD
Identifiant IRDPAR00020246
Lien permanenthttp://www.documentation.ird.fr/hor/PAR00020246

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