@article{PAR00020246,
  title = {{A}ssessment of biotechnological potentials of strains isolated from repasso olive pomace in {T}unisia},
  author = {{J}meii, {L}. and {S}oufi, {L}. and {A}bid, {N}. and {M}ahjoubi, {M}. and {R}oussos, {S}evastianos and {O}uzari, {H}. {I}. and {C}herif, {A}. and {G}arna, {H}.},
  editor = {},
  language = {{ENG}},
  abstract = {{P}urpose {T}he agri-food industry generates significant amounts of byproducts, among them repasso olive pomace ({ROP}) which are rejected and can constitute a serious environmental problem. {O}ur study aimed to investigate the diversity of microbiota isolated from {ROP} and screen for their biotechnological potentials. {M}ethods {A} collection of 102 strains (88 bacteria and 14 fungi) was obtained from a {ROP} sample. {T}he diversity of the bacterial collection was evaluated by amplification of the internal transcribed spacers between the 16{S} and the 23{S} r{RNA} genes ({ITS}-{PCR}) and by 16{S} r{RNA} sequencing. {F}ungal identification was performed by polymerase chain reaction ({PCR}) amplification of the {ITS}1-5.8{S}-{ITS}2 ribosomal {DNA} region. {T}he specific enzymatic screening of the detected microorganisms was tested. {R}esult {P}artial 16{S} r{RNA} gene sequencing performed on 44 isolates showed a high level of identity with known strains. {F}ungal strains identification showed that they belong to four families: {T}richocomaceae, {P}leurostomataceae, {M}ucoraceae, and {B}ionectriaceae. {O}ur results demonstrated that {G}ram-positive bacteria were mostly active, particularly for protease, lipase, amylase, cellulase laccase, and for biosurfactant production. {F}rom the 88 isolated bacteria, {F}irmicutes were the most prevalent and microdiverse. {B}acillus and {P}aenibacillus, together with some other {G}ram-negative bacteria such as {P}seudocitrobacter anthropi, and {A}cinetobacter johnsonii showed significant hydrolytic activities and biosurfactant production. {T}he 14 isolated fungi showed a high capacity of enzyme production. {T}his is the first study in {T}unisia describing the microbial diversity in {ROP} as well as the isolation of {B}acillus mojavensis producing lipase. {C}onclusion {M}icroorganisms especially fungi present in the repasso olive cake produce diverse hydrolytic enzymes of industrial interest.},
  keywords = {{B}acteria ; {F}ungi ; {ROP} ; {B}iotechnological activities ; {TUNISIE}},
  booktitle = {},
  journal = {{A}nnals of {M}icrobiology},
  volume = {69},
  numero = {11},
  pages = {1177--1190},
  ISSN = {1590-4261},
  year = {2019},
  DOI = {10.1007/s13213-019-01499-y},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00020246},
}