Federici F., Dupuy L., Laplaze Laurent, Heisler M., Haseloff J. (2012). Integrated genetic and computation methods for in planta cytometry. Nature Methods, 9 (5), p. 483-U104. ISSN 1548-7091.
Titre du document
Integrated genetic and computation methods for in planta cytometry
Federici F., Dupuy L., Laplaze Laurent, Heisler M., Haseloff J.
Source
Nature Methods, 2012,
9 (5), p. 483-U104 ISSN 1548-7091
We present the coupled use of specifically localized fluorescent gene markers and image processing for automated quantitative analysis of cell growth and genetic activity across living plant tissues. We used fluorescent protein markers to identify cells, create seeds and boundaries for the automatic segmentation of cell geometries and ratiometrically measure gene expression cell by cell in Arabidopsis thaliana.
