@article{PAR00008940, title = {{I}ntegrated genetic and computation methods for in planta cytometry}, author = {{F}ederici, {F}. and {D}upuy, {L}. and {L}aplaze, {L}aurent and {H}eisler, {M}. and {H}aseloff, {J}.}, editor = {}, language = {{ENG}}, abstract = {{W}e present the coupled use of specifically localized fluorescent gene markers and image processing for automated quantitative analysis of cell growth and genetic activity across living plant tissues. {W}e used fluorescent protein markers to identify cells, create seeds and boundaries for the automatic segmentation of cell geometries and ratiometrically measure gene expression cell by cell in {A}rabidopsis thaliana.}, keywords = {}, booktitle = {}, journal = {{N}ature {M}ethods}, volume = {9}, numero = {5}, pages = {483--{U}104}, ISSN = {1548-7091}, year = {2012}, DOI = {10.1038/nmeth.1940}, URL = {https://www.documentation.ird.fr/hor/{PAR}00008940}, }