Evaluating resistance to Bt toxin Cry1Ab by F-2 screen in European populations of Ostrinia nubilalis (Lepidoptera : Crambidae)

2010

Article référencé dans le Web of Science WOS:000282793000037

Engels H., Bourguet Denis, Cagan L., Manachini B., Schuphan I., Stodola T. J., Micoud A., Brazier C., Mottet C., Andow D. A.

Journal of Economic Entomology, 2010, 103 (5), p. 1803-1809 ISSN 0022-0493

The large-scale cultivation of transgenic crops producing Bacillus thuringiensis (Bt) toxins have already lead to the evolution of Bt resistance in some pest populations targeted by these crops. We used the F-2 screening method for further estimating the frequency of resistance alleles of the European corn borer, Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae), to Bt maize, Zea mays L., producing the Cry1Ab toxin. In France, Germany, and Italy, 784, 455, and 80 lines of European corn borer were screened for resistance to Mon810 maize, respectively. In Slovakia, 26 lines were screened for resistance to the Cry1Ab toxin. The cost of F-2 screen performed in the four countries varied from US300 to 1,300 per line screened. The major difference in cost was mostly due to a severe loss of univoltine lines during the screen in Germany and Slovakia. In none of the screened lines did we detect alleles conferring resistance to Mon810 maize or to the Cry1Ab toxin. The frequency of resistance alleles were <1.0 x 10(-3), <1.6 x 10(-3), <9.2 x 10(-3), and <2.6 x 10(-2) in France, Germany, Italy, and Slovakia, with 95% probability, respectively. The average detection probability over all lines was Making the assumption that European corn borer populations in these countries belong to the same genetic entity, the frequency of alleles conferring resistance to the Cry1Ab produced by the Mon810 maize in western and central Europe was 1.0 x 10(-4), with a 95% confidence interval of 0-3.0 x 10(-4).

Sciences du monde végétal [076]

PAR00006302