Ibrahim H., Bouajila J., Siri N., Rozing G., Nepveu Françoise, Couderc F. (2007). Very small injected samples to study chloroquine and quinine in human serum using capillary-LC and native fluorescence. Journal of Chromatography B Analytical Technologies in the Biomedical and Life Sciences, 850 (1-2), p. 481-487. ISSN 1570-0232.
Titre du document
Very small injected samples to study chloroquine and quinine in human serum using capillary-LC and native fluorescence
Ibrahim H., Bouajila J., Siri N., Rozing G., Nepveu Françoise, Couderc F.
Source
Journal of Chromatography B Analytical Technologies in the Biomedical and Life Sciences, 2007,
850 (1-2), p. 481-487 ISSN 1570-0232
A comparison between HPLC with conventional fluorescence detection and capillary-LC (mu HPLC) with native laser-induced fluorescence (LIF) detection was done to determine chloroquine (CQ) and quinine (Q) in human serum. HPLC experiments were run with parameters of the conventional fluorimeter set at the highest level of sensitivity. Results were compared with those obtained on mu HPLC coupled to a ZETALIF (He-Cd 325 nm) detector which provided a 50-fold increase in sensitivity. In mu HPLC-LIF injection volumes were 200 nL instead of 10 mu L in conventional HPLC. The separation was completed within 3 min (6 min on HPLC). The limit of detection on mu HPLC-LIF was 1.9 and 1.3 fmol for CQ and Q, respectively. Both experiments were validated on serum samples. The mean recovery was more than 95% for CQ and Q. The intra-and inter-day precision and accuracy were found to be within the acceptable limits (< 10%).
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
