@article{PAR00001750,
  title = {{V}ery small injected samples to study chloroquine and quinine in human serum using capillary-{LC} and native fluorescence},
  author = {{I}brahim, {H}. and {B}ouajila, {J}. and {S}iri, {N}. and {R}ozing, {G}. and {N}epveu, {F}ran{\c{c}}oise and {C}ouderc, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} comparison between {HPLC} with conventional fluorescence detection and capillary-{LC} (mu {HPLC}) with native laser-induced fluorescence ({LIF}) detection was done to determine chloroquine ({CQ}) and quinine ({Q}) in human serum. {HPLC} experiments were run with parameters of the conventional fluorimeter set at the highest level of sensitivity. {R}esults were compared with those obtained on mu {HPLC} coupled to a {ZETALIF} ({H}e-{C}d 325 nm) detector which provided a 50-fold increase in sensitivity. {I}n mu {HPLC}-{LIF} injection volumes were 200 n{L} instead of 10 mu {L} in conventional {HPLC}. {T}he separation was completed within 3 min (6 min on {HPLC}). {T}he limit of detection on mu {HPLC}-{LIF} was 1.9 and 1.3 fmol for {CQ} and {Q}, respectively. {B}oth experiments were validated on serum samples. {T}he mean recovery was more than 95% for {CQ} and {Q}. {T}he intra-and inter-day precision and accuracy were found to be within the acceptable limits (< 10%).},
  keywords = {mu {HPLC} ; laser induced fluorescence detection ; chloroquine ; quinine},
  booktitle = {},
  journal = {{J}ournal of {C}hromatography {B} {A}nalytical {T}echnologies in the {B}iomedical and {L}ife {S}ciences},
  volume = {850},
  numero = {1-2},
  pages = {481--487},
  ISSN = {1570-0232},
  year = {2007},
  DOI = {10.1016/j.jchromb.2006.12.028},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00001750},
}