Nurtop E., Villarroel P. M. S., Pastorino B., Ninove L., Drexler J. F., Roca Y., Gake B., Dubot Pérès Audrey, Grard G., Peyrefitte C., Priet S., Lamballerie X. de, Gallian P. (2018). Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies [+ Correction 2019, vol 16, 1 p.]. Virology Journal, 15, art. 192 [6 p.+ correction]. ISSN 1743-422X.
Titre du document
Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies [+ Correction 2019, vol 16, 1 p.]
Année de publication
2018
Auteurs
Nurtop E., Villarroel P. M. S., Pastorino B., Ninove L., Drexler J. F., Roca Y., Gake B., Dubot Pérès Audrey, Grard G., Peyrefitte C., Priet S., Lamballerie X. de, Gallian P.
Source
Virology Journal, 2018,
15, art. 192 [6 p.+ correction] ISSN 1743-422X
Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies.
Plan de classement
Santé : généralités [050]
;
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
MARTINIQUE ; GUADELOUPE ; FRANCE ; CAMEROUN
Localisation
Fonds IRD [F B010074862]
Identifiant IRD
fdi:010074862
