%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Nurtop, E.
%A Villarroel, P. M. S.
%A Pastorino, B.
%A Ninove, L.
%A Drexler, J. F.
%A Roca, Y.
%A Gake, B.
%A Dubot Pérès, Audrey
%A Grard, G.
%A Peyrefitte, C.
%A Priet, S.
%A Lamballerie, X. de
%A Gallian, P.
%T Combination of ELISA screening and seroneutralisation tests to expedite Zika virus seroprevalence studies [+ Correction 2019, vol 16, 1 p.]
%D 2018
%L fdi:010074862
%G ENG
%J Virology Journal
%@ 1743-422X
%K Zika virus ; Seroepidemiology ; Virus neutralization test
%K MARTINIQUE ; GUADELOUPE ; FRANCE ; CAMEROUN
%M ISI:000454444000001
%P art. 192 [6 + correction]
%R 10.1186/s12985-018-1105-5
%U https://www.documentation.ird.fr/hor/fdi:010074862
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers19-01/010074862.pdf
%V 15
%W Horizon (IRD)
%X Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies.
%$ 052 ; 050