Pallach M., Marchetti R., Di Lorenzo F., Fabozzi A., Giraud Eric, Gully Djamel, Paduano L., Molinaro A., D'Errico G., Silipo A. (2018). Zymomonas mobilis exopolysaccharide structure and role in high ethanol tolerance. Carbohydrate Polymers, 201, p. 293-299. ISSN 0144-8617.
Titre du document
Zymomonas mobilis exopolysaccharide structure and role in high ethanol tolerance
Pallach M., Marchetti R., Di Lorenzo F., Fabozzi A., Giraud Eric, Gully Djamel, Paduano L., Molinaro A., D'Errico G., Silipo A.
Source
Carbohydrate Polymers, 2018,
201, p. 293-299 ISSN 0144-8617
Bacterial cell surface exopolysaccharides (EPS) provide a protective barrier from the external milieu and participate in host-environment interactions. Zymomonas mobilis, an ethanologenic Gram negative bacterium, is used by the industry in bio-ethanol production process, due to its extraordinary resistance to a highly ethanolic environment. We found that Z. mobilis produces a mixture of two EPSs, an [alpha - (1 -> 6)-D-Manp] mannose homopolymer and a galactose containing polysaccharide: [-> 2)-beta-D-Galf-(1 -> 3)-beta-D-Galp-(1 ->](n). A physicochemical study, conducted with diffusion-ordered spectroscopy (DOSY) and Dynamic Light Scattering (DLS), allowed to demonstrate that, differently from the majority of polysaccharides, ethanol is a good solvent for the galactose containing EPS, revealing that its chemical structure is specifically connected with the Zymomonas mobilis high ethanol tolerance.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Biotechnologies [084]
