%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Pallach, M.
%A Marchetti, R.
%A Di Lorenzo, F.
%A Fabozzi, A.
%A Giraud, Eric
%A Gully, Djamel
%A Paduano, L.
%A Molinaro, A.
%A D'Errico, G.
%A Silipo, A.
%T Zymomonas mobilis exopolysaccharide structure and role in high ethanol tolerance
%D 2018
%L fdi:010074046
%G ENG
%J Carbohydrate Polymers
%@ 0144-8617
%K Zymomonas mobilis ; Exopolysaccharide ; NMR spectroscopy ; DLS ; DOSY ; Ethanologenic bacteria
%M ISI:000445030100031
%P 293-299
%R 10.1016/j.carbpol.2018.08.072
%U https://www.documentation.ird.fr/hor/fdi:010074046
%> https://www.documentation.ird.fr/intranet/publi/2018/10/010074046.pdf
%V 201
%W Horizon (IRD)
%X Bacterial cell surface exopolysaccharides (EPS) provide a protective barrier from the external milieu and participate in host-environment interactions. Zymomonas mobilis, an ethanologenic Gram negative bacterium, is used by the industry in bio-ethanol production process, due to its extraordinary resistance to a highly ethanolic environment. We found that Z. mobilis produces a mixture of two EPSs, an [alpha - (1 -> 6)-D-Manp] mannose homopolymer and a galactose containing polysaccharide: [-> 2)-beta-D-Galf-(1 -> 3)-beta-D-Galp-(1 ->](n). A physicochemical study, conducted with diffusion-ordered spectroscopy (DOSY) and Dynamic Light Scattering (DLS), allowed to demonstrate that, differently from the majority of polysaccharides, ethanol is a good solvent for the galactose containing EPS, revealing that its chemical structure is specifically connected with the Zymomonas mobilis high ethanol tolerance.
%$ 020 ; 084