@article{fdi:010074046, title = {{Z}ymomonas mobilis exopolysaccharide structure and role in high ethanol tolerance}, author = {{P}allach, {M}. and {M}archetti, {R}. and {D}i {L}orenzo, {F}. and {F}abozzi, {A}. and {G}iraud, {E}ric and {G}ully, {D}jamel and {P}aduano, {L}. and {M}olinaro, {A}. and {D}'{E}rrico, {G}. and {S}ilipo, {A}.}, editor = {}, language = {{ENG}}, abstract = {{B}acterial cell surface exopolysaccharides ({EPS}) provide a protective barrier from the external milieu and participate in host-environment interactions. {Z}ymomonas mobilis, an ethanologenic {G}ram negative bacterium, is used by the industry in bio-ethanol production process, due to its extraordinary resistance to a highly ethanolic environment. {W}e found that {Z}. mobilis produces a mixture of two {EPS}s, an [alpha - (1 -> 6)-{D}-{M}anp] mannose homopolymer and a galactose containing polysaccharide: [-> 2)-beta-{D}-{G}alf-(1 -> 3)-beta-{D}-{G}alp-(1 ->](n). {A} physicochemical study, conducted with diffusion-ordered spectroscopy ({DOSY}) and {D}ynamic {L}ight {S}cattering ({DLS}), allowed to demonstrate that, differently from the majority of polysaccharides, ethanol is a good solvent for the galactose containing {EPS}, revealing that its chemical structure is specifically connected with the {Z}ymomonas mobilis high ethanol tolerance.}, keywords = {{Z}ymomonas mobilis ; {E}xopolysaccharide ; {NMR} spectroscopy ; {DLS} ; {DOSY} ; {E}thanologenic bacteria}, booktitle = {}, journal = {{C}arbohydrate {P}olymers}, volume = {201}, numero = {}, pages = {293--299}, ISSN = {0144-8617}, year = {2018}, DOI = {10.1016/j.carbpol.2018.08.072}, URL = {https://www.documentation.ird.fr/hor/fdi:010074046}, }