Pan C., Liu J., Bi W. L., Chen H. Y., Engelmann Florent, Wang Q. C. (2018). Cryopreservation of small leaf squares-bearing adventitious buds of Lilium oriental hybrid 'Siberia' by vitrification. Plant Cell Tissue and Organ Culture, 133 (1), p. 159-164. ISSN 0167-6857.
Titre du document
Cryopreservation of small leaf squares-bearing adventitious buds of Lilium oriental hybrid 'Siberia' by vitrification
Pan C., Liu J., Bi W. L., Chen H. Y., Engelmann Florent, Wang Q. C.
Source
Plant Cell Tissue and Organ Culture, 2018,
133 (1), p. 159-164 ISSN 0167-6857
We report a new cryopreservation method for Lilium Oriental hybrid 'Siberia'. Adventitious buds were induced from leaf segments cultured for 12 days on adventitious bud induction medium composed of half-strength Murashige and Skoog medium (MS) supplemented with 1 mg L-1 alpha-naphthalene acetic acid and 0.5 mg L-1 thidiazuron. Small leaf squares (SLSs, 3 x 4 mm), each bearing at least one adventitious bud, were cut from leaf segments, precultured on medium with 0.5 M sucrose for 1 day, and then treated for 20 min with a loading solution containing 0.4 M sucrose and 2 M glycerol, followed by exposure to plant vitrification solution 2 for 7 h at 0 A degrees C. Dehydrated SLSs were directly immersed in liquid nitrogen for 1 h. Cryopreserved SLSs were re-warmed in MS medium containing 1.2 M sucrose for 20 min at room temperature, followed by post-thaw culture for recovery. With this procedure, 85% survival and 72% shoot regrowth were achieved following cryopreservation. The use of SLSs bearing adventitious buds for cryopreservation reported in the present study eliminates the time-consuming and labour-intensive step of shoot tip excision, and has great potential to facilitate cryopreservation in other plant species.
Plan de classement
Sciences du monde végétal [076]
;
Biotechnologies [084]
