%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Pan, C.
%A Liu, J.
%A Bi, W. L.
%A Chen, H. Y.
%A Engelmann, Florent
%A Wang, Q. C.
%T Cryopreservation of small leaf squares-bearing adventitious buds of Lilium oriental hybrid 'Siberia' by vitrification
%D 2018
%L fdi:010072490
%G ENG
%J Plant Cell Tissue and Organ Culture
%@ 0167-6857
%K Adventitious buds ; Cryopreservation ; Leaf segments ; Lilium ; Vitrification
%M ISI:000427403800015
%N 1
%P 159-164
%R 10.1007/s11240-017-1363-8
%U https://www.documentation.ird.fr/hor/fdi:010072490
%> https://www.documentation.ird.fr/intranet/publi/2018/04/010072490.pdf
%V 133
%W Horizon (IRD)
%X We report a new cryopreservation method for Lilium Oriental hybrid 'Siberia'. Adventitious buds were induced from leaf segments cultured for 12 days on adventitious bud induction medium composed of half-strength Murashige and Skoog medium (MS) supplemented with 1 mg L-1 alpha-naphthalene acetic acid and 0.5 mg L-1 thidiazuron. Small leaf squares (SLSs, 3 x 4 mm), each bearing at least one adventitious bud, were cut from leaf segments, precultured on medium with 0.5 M sucrose for 1 day, and then treated for 20 min with a loading solution containing 0.4 M sucrose and 2 M glycerol, followed by exposure to plant vitrification solution 2 for 7 h at 0 A degrees C. Dehydrated SLSs were directly immersed in liquid nitrogen for 1 h. Cryopreserved SLSs were re-warmed in MS medium containing 1.2 M sucrose for 20 min at room temperature, followed by post-thaw culture for recovery. With this procedure, 85% survival and 72% shoot regrowth were achieved following cryopreservation. The use of SLSs bearing adventitious buds for cryopreservation reported in the present study eliminates the time-consuming and labour-intensive step of shoot tip excision, and has great potential to facilitate cryopreservation in other plant species.
%$ 076 ; 084