@article{fdi:010072490,
  title = {{C}ryopreservation of small leaf squares-bearing adventitious buds of {L}ilium oriental hybrid '{S}iberia' by vitrification},
  author = {{P}an, {C}. and {L}iu, {J}. and {B}i, {W}. {L}. and {C}hen, {H}. {Y}. and {E}ngelmann, {F}lorent and {W}ang, {Q}. {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e report a new cryopreservation method for {L}ilium {O}riental hybrid '{S}iberia'. {A}dventitious buds were induced from leaf segments cultured for 12 days on adventitious bud induction medium composed of half-strength {M}urashige and {S}koog medium ({MS}) supplemented with 1 mg {L}-1 alpha-naphthalene acetic acid and 0.5 mg {L}-1 thidiazuron. {S}mall leaf squares ({SLS}s, 3 x 4 mm), each bearing at least one adventitious bud, were cut from leaf segments, precultured on medium with 0.5 {M} sucrose for 1 day, and then treated for 20 min with a loading solution containing 0.4 {M} sucrose and 2 {M} glycerol, followed by exposure to plant vitrification solution 2 for 7 h at 0 {A} degrees {C}. {D}ehydrated {SLS}s were directly immersed in liquid nitrogen for 1 h. {C}ryopreserved {SLS}s were re-warmed in {MS} medium containing 1.2 {M} sucrose for 20 min at room temperature, followed by post-thaw culture for recovery. {W}ith this procedure, 85% survival and 72% shoot regrowth were achieved following cryopreservation. {T}he use of {SLS}s bearing adventitious buds for cryopreservation reported in the present study eliminates the time-consuming and labour-intensive step of shoot tip excision, and has great potential to facilitate cryopreservation in other plant species.},
  keywords = {{A}dventitious buds ; {C}ryopreservation ; {L}eaf segments ; {L}ilium ; {V}itrification},
  booktitle = {},
  journal = {{P}lant {C}ell {T}issue and {O}rgan {C}ulture},
  volume = {133},
  numero = {1},
  pages = {159--164},
  ISSN = {0167-6857},
  year = {2018},
  DOI = {10.1007/s11240-017-1363-8},
  URL = {https://www.documentation.ird.fr/hor/fdi:010072490},
}