Sengvilaipaseuth O., Castonguay-Vanier J., Chanthongthip A., Phonemixay O., Thongpaseuth S., Vongsouvath M., Newton P. N., Bharucha T., Dubot Pérès Audrey. (2017). Poor performance of two rapid immunochromatographic assays for anti-Japanese encephalitis virus immunoglobulin M detection in cerebrospinal fluid and serum from patients with suspected Japanese encephalitis virus infection in Laos. Transactions of the Royal Society of Tropical Medicine and Hygiene, 111 (8), p. 373-377. ISSN 0035-9203.
Titre du document
Poor performance of two rapid immunochromatographic assays for anti-Japanese encephalitis virus immunoglobulin M detection in cerebrospinal fluid and serum from patients with suspected Japanese encephalitis virus infection in Laos
Sengvilaipaseuth O., Castonguay-Vanier J., Chanthongthip A., Phonemixay O., Thongpaseuth S., Vongsouvath M., Newton P. N., Bharucha T., Dubot Pérès Audrey
Source
Transactions of the Royal Society of Tropical Medicine and Hygiene, 2017,
111 (8), p. 373-377 ISSN 0035-9203
Background: Japanese encephalitis virus (JEV) is a leading identified cause of encephalitis in Asia, often occurring in rural areas with poor access to laboratory diagnostics. We evaluated two rapid diagnostic tests (RDTs) for anti-JEV immunoglobulin M (IgM) detection. Methods: Consecutive cerebrospinal fluid and serum from 388 patients (704 samples) with suspected JEV infections admitted to six hospitals in Laos were tested with one of two SD-Bioline anti-JEV IgM RDTs and the World Health Organization standard anti-JEV IgM enzyme-linked immunosorbent assay (ELISA; Panbio Japanese Encephalitis-Dengue IgM Combo ELISA. Results and Conclusions: The performance of both RDTs showed strikingly low sensitivity in comparison to anti-JEV IgM antibody capture ELISA (2.1-51.4%), suggesting low sensitivity of the RDTs. We highlight the fundamental prerequisite to validate RDTs prior to use to ensure that they meet standards for testing.
