%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Sengvilaipaseuth, O.
%A Castonguay-Vanier, J.
%A Chanthongthip, A.
%A Phonemixay, O.
%A Thongpaseuth, S.
%A Vongsouvath, M.
%A Newton, P. N.
%A Bharucha, T.
%A Dubot Pérès, Audrey
%T Poor performance of two rapid immunochromatographic assays for anti-Japanese encephalitis virus immunoglobulin M detection in cerebrospinal fluid and serum from patients with suspected Japanese encephalitis virus infection in Laos
%D 2017
%L fdi:010071960
%G ENG
%J Transactions of the Royal Society of Tropical Medicine and Hygiene
%@ 0035-9203
%K Central nervous system infection ; Immunoassays ; Japanese encephalitis virus ; Laos ; Rapid diagnostic tests ; South-East Asia
%K LAOS
%M ISI:000418593000008
%N 8
%P 373-377
%R 10.1093/trstmh/trx067
%U https://www.documentation.ird.fr/hor/fdi:010071960
%> https://www.documentation.ird.fr/intranet/publi/2018/01/010071960.pdf
%V 111
%W Horizon (IRD)
%X Background: Japanese encephalitis virus (JEV) is a leading identified cause of encephalitis in Asia, often occurring in rural areas with poor access to laboratory diagnostics. We evaluated two rapid diagnostic tests (RDTs) for anti-JEV immunoglobulin M (IgM) detection. Methods: Consecutive cerebrospinal fluid and serum from 388 patients (704 samples) with suspected JEV infections admitted to six hospitals in Laos were tested with one of two SD-Bioline anti-JEV IgM RDTs and the World Health Organization standard anti-JEV IgM enzyme-linked immunosorbent assay (ELISA; Panbio Japanese Encephalitis-Dengue IgM Combo ELISA. Results and Conclusions: The performance of both RDTs showed strikingly low sensitivity in comparison to anti-JEV IgM antibody capture ELISA (2.1-51.4%), suggesting low sensitivity of the RDTs. We highlight the fundamental prerequisite to validate RDTs prior to use to ensure that they meet standards for testing.
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