@article{fdi:010071960, title = {{P}oor performance of two rapid immunochromatographic assays for anti-{J}apanese encephalitis virus immunoglobulin {M} detection in cerebrospinal fluid and serum from patients with suspected {J}apanese encephalitis virus infection in {L}aos}, author = {{S}engvilaipaseuth, {O}. and {C}astonguay-{V}anier, {J}. and {C}hanthongthip, {A}. and {P}honemixay, {O}. and {T}hongpaseuth, {S}. and {V}ongsouvath, {M}. and {N}ewton, {P}. {N}. and {B}harucha, {T}. and {D}ubot {P}{\'e}r{\`e}s, {A}udrey}, editor = {}, language = {{ENG}}, abstract = {{B}ackground: {J}apanese encephalitis virus ({JEV}) is a leading identified cause of encephalitis in {A}sia, often occurring in rural areas with poor access to laboratory diagnostics. {W}e evaluated two rapid diagnostic tests ({RDT}s) for anti-{JEV} immunoglobulin {M} ({I}g{M}) detection. {M}ethods: {C}onsecutive cerebrospinal fluid and serum from 388 patients (704 samples) with suspected {JEV} infections admitted to six hospitals in {L}aos were tested with one of two {SD}-{B}ioline anti-{JEV} {I}g{M} {RDT}s and the {W}orld {H}ealth {O}rganization standard anti-{JEV} {I}g{M} enzyme-linked immunosorbent assay ({ELISA}; {P}anbio {J}apanese {E}ncephalitis-{D}engue {I}g{M} {C}ombo {ELISA}. {R}esults and {C}onclusions: {T}he performance of both {RDT}s showed strikingly low sensitivity in comparison to anti-{JEV} {I}g{M} antibody capture {ELISA} (2.1-51.4%), suggesting low sensitivity of the {RDT}s. {W}e highlight the fundamental prerequisite to validate {RDT}s prior to use to ensure that they meet standards for testing.}, keywords = {{C}entral nervous system infection ; {I}mmunoassays ; {J}apanese encephalitis virus ; {L}aos ; {R}apid diagnostic tests ; {S}outh-{E}ast {A}sia ; {LAOS}}, booktitle = {}, journal = {{T}ransactions of the {R}oyal {S}ociety of {T}ropical {M}edicine and {H}ygiene}, volume = {111}, numero = {8}, pages = {373--377}, ISSN = {0035-9203}, year = {2017}, DOI = {10.1093/trstmh/trx067}, URL = {https://www.documentation.ird.fr/hor/fdi:010071960}, }