Braidy N., Matin A., Rossi F., Chinain M., Laurent Dominique, Guillemin G. J. (2014). Neuroprotective effects of rosmarinic acid on ciguatoxin in primary human neurons. Neurotoxicity Research, 25 (2), p. 226-234. ISSN 1029-8428.
Titre du document
Neuroprotective effects of rosmarinic acid on ciguatoxin in primary human neurons
Braidy N., Matin A., Rossi F., Chinain M., Laurent Dominique, Guillemin G. J.
Source
Neurotoxicity Research, 2014,
25 (2), p. 226-234 ISSN 1029-8428
Ciguatoxin (CTX), is a toxic compound produced by microalgae (dinoflagellate) Gambierdiscus spp., and is bio-accumulated and bio-transformed through the marine food chain causing neurological deficits. To determine the mechanism of CTX-mediated cytotoxicity in human neurons, we measured extracellular lactate dehydrogenase (LDH) activity, intracellular levels of nicotinamide adenine dinucleotide (NAD(+)) and H2AX phosphorylation at serine 139 as a measure for DNA damage in primary cultures of human neurons treated with Pacific (P)-CTX-1B and P-CTX-3C. We found these marine toxins can induce a time and dose-dependent increase in extracellular LDH activity, with a concomitant decline in intracellular NAD(+) levels and increased DNA damage at the concentration range of 5-200 nM. We also showed that pre- and post-treatment with rosmarinic acid (RA), the active constituent of the Heliotropium foertherianum (Boraginaceae) can attenuate CTX-mediated neurotoxicity. These results further highlight the potential of RA in the treatment of CTX-induced neurological deficits.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Santé : généralités [050]
;
Sciences du monde végétal [076]
