%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Braidy, N.
%A Matin, A.
%A Rossi, F.
%A Chinain, M.
%A Laurent, Dominique
%A Guillemin, G. J.
%T Neuroprotective effects of rosmarinic acid on ciguatoxin in primary human neurons
%D 2014
%L fdi:010061478
%G ENG
%J Neurotoxicity Research
%@ 1029-8428
%K Ciguatera ; Ciguatoxin ; DNA damage ; NAD(+) ; Neurotoxicity ; Rosmarinic acid
%M ISI:000329326900008
%N 2
%P 226-234
%R 10.1007/s12640-013-9429-9
%U https://www.documentation.ird.fr/hor/fdi:010061478
%> https://www.documentation.ird.fr/intranet/publi/2014/02/010061478.pdf
%V 25
%W Horizon (IRD)
%X Ciguatoxin (CTX), is a toxic compound produced by microalgae (dinoflagellate) Gambierdiscus spp., and is bio-accumulated and bio-transformed through the marine food chain causing neurological deficits. To determine the mechanism of CTX-mediated cytotoxicity in human neurons, we measured extracellular lactate dehydrogenase (LDH) activity, intracellular levels of nicotinamide adenine dinucleotide (NAD(+)) and H2AX phosphorylation at serine 139 as a measure for DNA damage in primary cultures of human neurons treated with Pacific (P)-CTX-1B and P-CTX-3C. We found these marine toxins can induce a time and dose-dependent increase in extracellular LDH activity, with a concomitant decline in intracellular NAD(+) levels and increased DNA damage at the concentration range of 5-200 nM. We also showed that pre- and post-treatment with rosmarinic acid (RA), the active constituent of the Heliotropium foertherianum (Boraginaceae) can attenuate CTX-mediated neurotoxicity. These results further highlight the potential of RA in the treatment of CTX-induced neurological deficits.
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