Publications des scientifiques de l'IRD

Barraco Giuseppe, Sylvestre Isabelle, Iapichino G., Engelmann Florent. (2011). Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification. Scientia Horticulturae, 130 (1), p. 309-313. ISSN 0304-4238.

Titre du document
Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification
Année de publication
2011
Type de document
Article référencé dans le Web of Science WOS:000294942900045
Auteurs
Barraco Giuseppe, Sylvestre Isabelle, Iapichino G., Engelmann Florent
Source
Scientia Horticulturae, 2011, 130 (1), p. 309-313 ISSN 0304-4238
In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreserved using the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips was possible only when samples were pretreated for 16 h in liquid medium with 0.3 M sucrose, then for 5 h in liquid medium with 0.7 M sucrose before performing the cryopreservation protocol. Optimal conditions included treatment for 20 min in a loading solution containing 1.9 M glycerol + 0.5 M sucrose, treatment with vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solution A9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapid cooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloading solution containing 1.2 M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tips was achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation.
Plan de classement
Sciences du monde végétal [076] ; Biotechnologies [084]
Localisation
Fonds IRD [F B010053841]
Identifiant IRD
fdi:010053841
Contact