%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Barraco, Giuseppe
%A Sylvestre, Isabelle
%A Iapichino, G.
%A Engelmann, Florent
%T Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification
%D 2011
%L fdi:010053841
%G ENG
%J Scientia Horticulturae
%@ 0304-4238
%K Cryopreservation ; Droplet-vitrification ; Limonium serotinum ; Statice ; Sucrose pretreatment ; Vitrification solution
%M ISI:000294942900045
%N 1
%P 309-313
%R 10.1016/j.scienta.2011.07.001
%U https://www.documentation.ird.fr/hor/fdi:010053841
%> https://www.documentation.ird.fr/intranet/publi/2011/10/010053841.pdf
%V 130
%W Horizon (IRD)
%X In this study in vitro shoot tips of a Sicilian genotype of Limonium serotinum were successfully cryopreserved using the droplet-vitrification technique. Growth recovery of cryopreserved shoot tips was possible only when samples were pretreated for 16 h in liquid medium with 0.3 M sucrose, then for 5 h in liquid medium with 0.7 M sucrose before performing the cryopreservation protocol. Optimal conditions included treatment for 20 min in a loading solution containing 1.9 M glycerol + 0.5 M sucrose, treatment with vitrification solution B5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solution A9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapid cooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloading solution containing 1.2 M sucrose. Under these conditions, 37% recovery of cryopreserved shoot tips was achieved. Regrowth of cryopreserved samples was slow but always direct, without callus formation.
%$ 076 ; 084