@article{fdi:010053841,
  title = {{C}ryopreservation of {L}imonium serotinum apical meristems from in vitro plantlets using droplet-vitrification},
  author = {{B}arraco, {G}iuseppe and {S}ylvestre, {I}sabelle and {I}apichino, {G}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n this study in vitro shoot tips of a {S}icilian genotype of {L}imonium serotinum were successfully cryopreserved using the droplet-vitrification technique. {G}rowth recovery of cryopreserved shoot tips was possible only when samples were pretreated for 16 h in liquid medium with 0.3 {M} sucrose, then for 5 h in liquid medium with 0.7 {M} sucrose before performing the cryopreservation protocol. {O}ptimal conditions included treatment for 20 min in a loading solution containing 1.9 {M} glycerol + 0.5 {M} sucrose, treatment with vitrification solution {B}5 (glycerol 40.0%, sucrose 40.0%, w/v) for 60 and 90 min or vitrification solution {A}9 (glycerol 30.0%, dimethylsulfoxide 20.0%, ethylene glycol 20.0%, sucrose 15.0%) for 20 min, rapid cooling in minute droplets of vitrification solution, rapid rewarming by immersion for 20 min in unloading solution containing 1.2 {M} sucrose. {U}nder these conditions, 37% recovery of cryopreserved shoot tips was achieved. {R}egrowth of cryopreserved samples was slow but always direct, without callus formation.},
  keywords = {{C}ryopreservation ; {D}roplet-vitrification ; {L}imonium serotinum ; {S}tatice ; {S}ucrose pretreatment ; {V}itrification solution},
  booktitle = {},
  journal = {{S}cientia {H}orticulturae},
  volume = {130},
  numero = {1},
  pages = {309--313},
  ISSN = {0304-4238},
  year = {2011},
  DOI = {10.1016/j.scienta.2011.07.001},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053841},
}