Gadea G., Bos S., Krejbich-Trotot P., Clain E., Viranaicken W., El-Kalamouni C., Mavingui Patrick, Despres P. (2016). A robust method for the rapid generation of recombinant Zika virus expressing the GFP reporter gene. Virology, 497, p. 157-162. ISSN 0042-6822.
Titre du document
A robust method for the rapid generation of recombinant Zika virus expressing the GFP reporter gene
Gadea G., Bos S., Krejbich-Trotot P., Clain E., Viranaicken W., El-Kalamouni C., Mavingui Patrick, Despres P.
Source
Virology, 2016,
497, p. 157-162 ISSN 0042-6822
Zika virus (ZIKV) infection is a major public health problem with severe human congenital and neurological anomalies. The screening of anti-ZIKV compounds and neutralizing antibodies needs reliable and rapid virus-based assays. Here, we described a convenient method leading to the rapid production of molecular clones of ZIKV. To generate a molecular clone of ZIKV strain MR766(NIID), the viral genome was directly assembled into Vero cells after introduction of four overlapping synthetic fragments that cover the full-length genomic RNA sequence. Such strategy has allowed the production of a recombinant ZIKV expressing the GFP reporter gene that is stable over two culturing rounds on Vero cells. Our data demonstrate that the ZIKV reporter virus is a very reliable GFP-based tool for analyzing viral growth and measuring the neutralizing antibody as well as rapid screening of antiviral effect of different classes of inhibitors.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Entomologie médicale / Parasitologie / Virologie [052]
